A synthetic probe STR 14C19, detects a new polymorphic locus at 16pter (D16S282).
نویسندگان
چکیده
Detection by PCR of the VNTR polymorphism at D4S95 Bernice A.AIIitto, Glenn T.Horn, Michael R.AItherr, Brenda Richards, Andrea I.McClatchey, John J.Wasmuth and James F.Gusella* Neurogenetics Laboratory, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, MA 02114, department of Human Genetics, Integrated Genetics, One Mountain Road, Framingham, MA 01752 and department of Biological Chemistry, University of California, Irvine, CA 92717, USA
منابع مشابه
Development of Highly Polymorphic Pentanucleotide Tandem Repeat Loci with Low Stutter
INTRODUCTION All eukaryotic genomes contain regions of simple repetitive DNA, called short tandem repeats (STR*) or microsatellites, which consist of tandem repeats of a small number of bases (1-3). The number of repeats at a STR locus can be highly variable among individuals, resulting in length polymorphisms that can be detected by relatively simple PCR-based assays. Thousands of these highly...
متن کاملCharacterisation of short tandem repeats for genotyping Mycobacterium leprae.
OBJECTIVE Establish a typing system for Mycobacterium leprae based on polymorphic DNA structures known as short tandem repeats (STR). DESIGN Assess 16 polymorphic STR for sensitivity, specificity and reproducibility in standard assays using reference strains of M. leprae. RESULTS Primers for 16 STR loci were selected based on PCR product size and for their ability to sequence each STR locus...
متن کاملIdentification and characterization of the highly polymorphic locus D14S739 in the Han Chinese population
AIM To systemically select and evaluate short tandem repeats (STRs) on the chromosome 14 and obtain new STR loci as expanded genotyping markers for forensic application. METHODS STRs on the chromosome 14 were filtered from Tandem Repeats Database and further selected based on their positions on the chromosome, repeat patterns of the core sequences, sequence homology of the flanking regions, a...
متن کاملHaplotype data for 23 Y-chromosome markers in a reference sample from Bosnia and Herzegovina
AIM To detect polymorphisms of 23 Y-chromosomal short tandem repeat (STR) loci, including 6 new loci, in a reference database of male population of Bosnia and Herzegovina, as well as to assess the importance of increasing the number of Y-STR loci utilized in forensic DNA analysis. METHODS The reference sample consisted of 100 healthy, unrelated men originating from Bosnia and Herzegovina. Sam...
متن کاملStuI RFLP in the human ABL locus.
Source/Description: A 3.6 kb EcoRI fragment (lambda ABL3) containing portions of exons 7 and 8 and the intervening intron of the ABL oncogene was isolated from a chromosome 9-specific library (ATCC # LA09NS01) using a 1.7 kb Abelson murine leukemia virus probe (v-abl, ONCOR, Inc.). A single copy 1.8 kb BglII fragment (ABL3) was derived from the original lambda clone and detects the StuI RFLP. P...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Nucleic acids research
دوره 19 14 شماره
صفحات -
تاریخ انتشار 1991